This fullcolor atlas will aid experimental design and interpretation in these areas by providing a fundamental understanding of zebrafish anatomy. The zebrafish anatomy ontology zfa is an obo foundry ontology that is used in conjunction with the zebrafish stage ontology zfs to describe the gross and cellular anatomy and development of the zebrafish, danio rerio, from single cell zygote to adult. Zebra fish, any member of either of two unrelated groups of fishes, the freshwater species in the genus brachydanio family cyprinidae. Anatomy atlases and resources zebrafish information network. Growth of the zebrafish anatomy ontology expanded to support adult morphology and dynamic changes in the early embryo ceri e. Blood exits the heart through the bulbus arteriosus and the ventral aorta then branches to the right and left into the mandibular first aortic arches. The zebrafish model organism database zfin uses the zfa and zfs to annotate phenotype and gene expression data from the. Highlights we describe a novel user friendly anatomical resource for zebrafish. On the home page menu click on search anatomical terms.
Users can view threedimensional fluorescent angiograms of any region of the embryo at any stage of development from 17 days postfertilization. Science education collection an introduction to the. About zfap the zebrafish anatomy portal allows users to. Wildcard searching if you want to search for multiple variations of a word, you can substitute a special symbol called a wildcard for one or more letters.
The zebrafish virtual atlas and the zebrafish anatomy project the zebrafish atlas contains 2 and 3dimensional, anatomical reference slides of zebrafish to support research and education worldwide serial histological images of wild type zebrafish are used for 3d reconstructions. Anatomy, animal gross anatomy, fish anatomy, gross anatomy. Normal anatomy and histology of the adult zebrafish. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical. Cyprinidae, which contains over 2,400 species, including the loveable goldfish. The course is held either in english or in german depending on. The zebrafish anatomy portal zfap is an anatomical resource for the zebrafish danio rerio containing 3d models of zebrafish covering the range of development from 24hrs until adulthood. The sequencing of its genome and the relative ease with which gene modifications can be performed have led to the creation of numerous human disease models that can be used for testing the potential and the toxicity.
Constantinescu, dvm, phd, drhc, a veterinary anatomist and medical illustrator at the university of missouricolumbia. Zebrafish get their name because they resemble zebras. Mar 09, 2016 the inner ear is a remarkably intricate structure able to detect sound, motion, and gravity. Search for annotated images illustrating structures of interest. Zebrafish study reveals first fine structure of a complete.
Atlas of zebrafish development download ebook pdf, epub. Provide a portal for improved searching of other information sources. Weinstein,1 laboratory of molecular genetics, nichd, nih, building 6b, room 309, 6 center drive. To only access descriptive diagrams detailing the wiring patterns of zebrafish vessels click the left button below. Feb 25, 2014 the zebrafish anatomy ontology zfa is an obo foundry ontology that is used in conjunction with the zebrafish stage ontology zfs to describe the gross and cellular anatomy and development of the zebrafish, danio rerio, from single cell zygote to adult. The 120 hr embryo dorsal view click on a number to follow the appropriate link lateral view. For example, mutant zebrafish are being utilized for testing drugs that will combat a range of human diseases, from alzheimers and cancer to kidney failure and congenital heart disease. Anterior is to left in this and other figures in the paper except where indicated. Animated images in the analysis of zebrafish behavior. Zebrafish vascular anatomy introduction of the interactive. Zebrafish danio rerio have become an increasingly popular genetic model over the past twenty years.
This method and the profound optical clarity of zebrafish embryos make it possible to view the entire developing vasculature with unprecedented resolution. Anatomical dissection of zebrafish brain development. Review 1 the behaviour and ecology of the zebrafish, danio rerio rowena spence1, gabriele gerlach2, christian lawrence3 and carl smith1 1 department of biology, university of leicester, university road, leicester, le1 7rh, uk. This chapter covers detail of materials and protocols that we employ to generate data for the atlas. Zfin the 120 hr embryo zebrafish information network. The advantages of zebrafish over classical vertebrate models are multifactorial and include high genetic and organ system homology to humans, high fecundity, external fertilization, ease of genetic manipulation, and. Probiotic modulation of the microbiotagutbrain axis and. An introduction to zebrafish jove journal of visualized. More precisely, zebrafish belong to the single largest vertebrate family. Danio rerio are among the smallest members of this family, with adults measuring 30 40 millimeters, or about 1. Zebrafishbrain information about the neuroanatomy of the developing zebrafish brain. Initially blood flows through a simple single circulatory loop 24 hpf overview.
A virtual slide, with capabilities to zoom and scroll, illustrates the term. A lifespan atlas of the zebrafish bioatlas bioatlas. The latest edition which is the second edition was published in 2009 by elsevier. Zebrafish anatomy and development ontology ncbo bioportal. Internal organs are illustrated in multiple dissections. The zebrafish has been shown to be an excellent vertebrate model for studying the roles of specific genes and signaling pathways. High resolution confocal imaging of intact brains in which.
We define seven broad peri ods of embryogenesisthe zygote, cleavage, blas. If you have ever heard of the show or watched it, you must know that it is aimed at medical students and it. The deltaa gene of zebrafish mediates lateral inhibition of hair cells in the inner ear and is regulated by pax2. Zebrafish study reveals first fine structure of a complete vertebrate brain 11 may 2017 zebrafish larva head in crosssection. This website is an online interactive atlas displaying in threedimensional detail the complete vascular anatomy of the developing zebrafish. Specification and morphogenesis of the zebrafish larval. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. This collection of sections through zebrafish embryos at four different stages of development is thought to provide some help to understand how the zebrafish embryo looks inside.
The visual system of zebrafish and its use to model human ocular. Thin section in araldite were stained with methylene blue. Thus, the primary form of mercury to which humans are exposed is methylmercury. Over the last two decades, zebrafish have been established as a genetically versatile model system for investigating many different aspects of vertebrate developmental biology. Use of zebrafish danio rerio embryos as a model to assess effects. Author links open overlay panel david salgado b christophe marcelle b peter d. Aug 27, 20 is an online neuroanatomical atlas of the embryonic zebrafish. This fullcolor atlas will aid experimental design and interpretation in these areas by providing a fundamental understanding of zebrafish. Click download or read online button to get atlas of zebrafish development book now. Aug 17, 2007 over the last two decades, zebrafish have been established as a genetically versatile model system for investigating many different aspects of vertebrate developmental biology. Provide the ability to search images by anatomical terms.
The drawings illustrate the critical embryonic stages and details the adult anatomy, including external views of the male and female lateral and male ventral, skeleton, viscera of the female and male lateral, and male ventral, internal organs, central nervous system, histological views of male and. The atlas uses highresolution confocal images and movies of transgenic lines to describe different brain structures. This site is like a library, use search box in the widget to get ebook that you want. Oct 15, 2019 the zebrafish virtual atlas and the zebrafish anatomy project the zebrafish atlas contains 2 and 3dimensional, anatomical reference slides of zebrafish to support research and education worldwide serial histological images of wild type zebrafish are used for 3d reconstructions. Development of utricular otoliths, but not saccular otoliths, is necessary for vestibular function and survival in zebrafish.
Neurochemical anatomy of the zebrafish retina as determined by immunocytochemistry. The study of zebrafish as a leading model organism for developmental biology is rapidly expanding. Grays anatomy for students pdf is a book written by adam mitchell, richard drake, and wayne vogl. Free anatomy and physiology textbook for download openstax. The development of tools to predict gene expression from literature analysis.
The vertebrate cornea is the transparent tissue covering the. Richardson technological innovation has helped the zebrafish embryo gain ground as a disease model and an assay system for drug screening. In our lab we use the zebrafish for cancer research, and it is the basis for this histological atlas. With the credentials of zebrafish as a developmental model now well recognized, the emerging new opportunity is the wider application of zebrafish biology to aspects of human disease modelling. For the first time, this atlas provides the research community with a complete reference for zebrafish anatomy spanning the early embryo all the way to adulthood. We have used confocal microangiography to examine and describe the vascular anatomy of the developing zebrafish, danio rerio. Online help keyboard shortcuts feed builder whats new. The gut microbiota plays a crucial role in the bidirectional gutbrain axis, a communication that integrates the gut and central nervous system cns activities.
Chemical anatomy of the zebrafish retina springerlink. Learn about human anatomy and physiology online by downloading openstaxs free anatomy and physiology book and using our accompanying study guide. A guide for the laboratory use of zebrafish danio rerio, eugene, university of oregon press. The sequencing of its genome and the relative ease with which gene modifications can be performed have led to the creation of numerous human disease models that can be used for testing the potential and the toxicity of new pharmaceutical compounds. Danio rerio commonly known as the zebrafish is a tropical fish belonging to the minnow family cyprinidae, commonly kept in aquaria and used for scientific research.
A novel integrated resource to facilitate zebrafish research. A structured controlled vocabulary of the anatomy and development of the zebrafish danio rerio. If you want to view 3d angiography images, click the right button below. Guidance on the housing and care of zebrafish, danio rerio. Although experimental analysis of the embryo and larva is extensive and the morphology has been well documented, descriptions of adult zebrafish anatomy and studies of development of the adult structures and organs, together with techniques for working with adults are lacking.
This collection of sections through zebrafish embryos at four different stages of. Neuroanatomy of the zebrafish brain a topological atlas mario f. The zebrafish international resource center is housed on the university of oregon campus in eugene. Although human pathologies have mostly been modeled using higher mammal systems such as mice, the lower vertebrate zebrafish has gained tremendous attention as a model system. Anatomy this poster portrays the critical embryonic stages and the details of adult anatomy, including aspects of sexual dimorphism. This section offers an interactive reference to each anatomical term identified in the zebrafish atlas. The growing interest in zebrafish research was paralleled by an increase in tools and methods.
The zebrafish model organism database zfin uses the zfa and zfs to annotate phenotype and gene expression data from the primary literature. The zebrafish model organism database zfin uses the zfa and zfs to annotate phenotype. An online database of zebrafish anatomy article pdf available in bmc biology 51. Pdf normal anatomy and histology of the adult zebrafish. Anatomy of the 24, 48, 72 and 120 hours zebrafish danio rerio embryo. For example, world war ii with quotes will give more precise results than world war ii without quotes. A molecular phenotype atlas of the zebrafish retina. Its mission is to provide a central repository for wildtype and mutant strains of zebrafish danio rerio and for materials and information about zebrafish research. The atlas spans the life of the zebrafish from embryo to. The zebrafish danio rerio is a valuable and common model for researchers working in the fields of genetics, oncology and developmental sciences. Normal table of postembryonic zebrafish development. The zebrafish model organism database zfin uses the zfa and zfs to annotate phenotype and gene expression data.
To view an anatomical part within the context of a virtual slide, choose a term from the list below. A structured controlled vocabulary of the anatomy and development of the zebrafish. This poster is a valuable reference tool, featuring illustrations on the zebrafish anatomy. Phrase searching you can use double quotes to search for a series of words in a particular order. Generation of disease models and drug screens shaukat ali, danielle l. In the following sections, we describe the anatomy and physiology of the zebrafish cornea and lens. During development of the zebrafish embryo, the ear undergoes dynamic morphogenesis from a simple epithelial vesicle into a complex labyrinth, consisting of three semicircular canals and three otolithic sensory organs, each with an array of differentiated cell types. Use the autocomplete tool to find the term that you want to use. The zebrafish anatomy poster drawings were done by gheorghe m. The behaviour and ecology of the zebrafish, danio rerio. Interestingly, unlike in prior studies where zebrafish embryos were exposed to higher doses of alcohol and for longer periods of alcohol administration e.
An atlas of embryonic and early larval development sumio isogai, masaharu horiguchi, and brant m. The zebrafish atlas of macroscopic and microscopic anatomy the zebrafish danio rerio is avaluable and common model for researchers working in the fields of genetics, oncology, and developmental sciences. Materials and zebrafish strains are distributed to the research community. Dissection of organs from the adult zebrafish protocol. Whilst the vascular anatomy of the embryonic fish, the anatomy of the embryonic and adult brain 11,12, and heart have been elegantly described, there is a need for a detailed record of zebrafish development that spans the entire life history of the organism and presents its anatomy. This links you to a page from which you can directly load different diagrams of vessels in embryos from 17 days old. Staging by externally visible anatomy of the living fish. Anatomy atlases and resources general information zfin. Methods to study the development, anatomy, and function of. For further description see schilling and kimmel 1997 and cubbage and mabee 1996.
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